Recombinant AMARV GP protein (His tag) (ab190126)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Tags: His tag C-Terminus
- Suitable for: WB, SDS-PAGE, ELISA
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Product name
Recombinant AMARV GP protein (His tag) -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Marburg virus -
Sequence
MKTTCLLISLILIQGVKTLPILEIASNIQPQNVDSVCSGTLQKTEDVHLM GFTLSGQKVADSPLEASKRW AFRAGVPPKNVEYTEGEEAKTCYNISVT DPSGKSLLLDPPTNIRDYPKCKTIHHIQGQNPHAQGIALHLW GAFFLY DRIASTTMYRGKVFTEGNIAAMIVNKTVHKMIFSRQGQGYRHMNLTSTNK YWTSSNGTQTNDTG CFGTLQEYNSTKNQTCAPSKKPLPLPTAHPEVKL TSTSTDATKLNTTDPNSDDEDLTTSGSGSGEQEPYT TSDAATKQGLSS TMPPTPSPQPSTPQQGGNNTNHSQGVVTEPGKTNTTAQPSMPPHNTTTIS TNNTSKHN LSTPSVPIQNATNYNTQSTAPENEQTSAPSKTTLLPTENP TTAKSTNSTKSPTTTVPNTTNKYSTSPSPT PDSTAQHLVYFRRKRNIL WREGDMFPFLDGLINAPIDFDPVPNTKTIFDESSSSGASAEEDQHASPNI SL TLSYFPKVNENTAHSGENENDCDAELRIWSVQEDDLAAGLSWIPFF GPGIEGLYTAGLIKNQNNLVCRLR RLANQTAKSLELLLRVTTEERTFS LINRHAIDFLLARWGGTCKVLGPDCCIGIEDLSRNISEQIDQIKKD EQ KEGTGWGLGGKWWTSDWGVLTNLGILLLLSIAVLIALSCICRIFTKYIG -
Predicted molecular weight
60 kDa including tags -
Tags
His tag C-Terminus -
Additional sequence information
Angola marburgvirus glycoprotein minus the transmembrane domain (MARV-Angola rGPdTM). The theoretical molecular weight of the protein is ~60 kDa including the His-tag, without glycosylation.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: PBS
PBS is supplemented with 10% glycerol, arginine and glutamic acid.
Images
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Western blot - Recombinant AMARV GP protein (His tag) (ab190126)All lanes : rabbit polyclonal antibody anti AMARV GP at 0.5 µg/ml
Lane 1 :Recombinant AMARV GP protein (His tag) (ab190126) at 0.1 µg
Lane 2 :Recombinant AMARV GP protein (His tag) (ab190126) at 0.5 µg
Lane 3 :Recombinant AMARV GP protein (His tag) (ab190126) at 1 µg
Secondary
All lanes : anti-rabbit IgG-HRP conjugate, followed by substrate.
Predicted band size: 74 kDa -
SDS-PAGE - Recombinant AMARV GP protein (His tag) (ab190126)SDS-PAGE analysis of 1 μg and 5 μg (lanes 1, 2 respectively) of ab190126 under denaturing and reducing conditions.
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ELISA - Recombinant AMARV GP protein (His tag) (ab190126)Plate was coated with ab190126 starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using one dilution of a positive control serum, followed with anti-IgG HRP conjugate and TM substrate. OD650 is reported. Background of ab190126 coated plate without positive control serum was 0.051 OD650.