HRP Anti-ADAR1 antibody [EPR7033] (ab206086)
![HRP Anti-ADAR1 antibody [EPR7033] (ab206086)](https://www.abcam.com/ps/products/206/ab206086/Images/ab206086-265662-WBab2060861.jpg "HRP Anti-ADAR1 antibody [EPR7033] (ab206086)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR7033] to ADAR1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
-
Product name
HRP Anti-ADAR1 antibody [EPR7033]
See all ADAR1 primary antibodies -
Description
HRP Rabbit monoclonal [EPR7033] to ADAR1 -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa and wildtype HAP1 whole cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7033 -
Isotype
IgG -
Research areas
Images
-
Western blot - HRP Anti-ADAR1 antibody [EPR7033] (ab206086)All lanes : HRP Anti-ADAR1 antibody [EPR7033] (ab206086) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : Wild-type HAP1 cell lysate at 20 µg
Lane 3 : ADAR1 knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 136 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab206086 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
![HRP Anti-ADAR1 antibody [EPR7033] (ab206086)](https://www.abcam.com/ps/products/206/ab206086/Images/ab206086-3-benefits-of-recombinant-antibodies.png)
HRP Anti-ADAR1 antibody [EPR7033] (ab206086)
