Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (ab251241)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16337] to MBNL3 - BSA and Azide free
  • Suitable for: WB, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-MBNL3 antibody [EPR16337] - BSA and Azide free
    See all MBNL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16337] to MBNL3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251241 is the carrier-free version of ab197590. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251241 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (ab251241)
    Western blot - Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (ab251241)
    Anti-MBNL3 antibody [EPR16337] (ab197590) at 1/1000 dilution + Human placenta lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa



    This data was developed using ab197590, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression is consistent with what has been described in the literature (PMID: 11929853).

  • Immunocytochemistry - Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (ab251241)
    Immunocytochemistry - Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (ab251241)
    This data was developed using ab197590, the same antibody clone in a different buffer formulation.ab197590 staining MBNL3 in JAR (human choriocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain. Control: PBS only
  • Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (ab251241)
    Anti-MBNL3 antibody [EPR16337] - BSA and Azide free (ab251241)

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