Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKN3 antibody [EPR3511(2)] - BSA and Azide free (ab242397)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3511(2)] to PKN3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PKN3 antibody [EPR3511(2)] - BSA and Azide free
    See all PKN3 primary antibodies

  • Description

    Rabbit monoclonal [EPR3511(2)] to PKN3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PC-3, Mouse spleen, Rat spleen lysates ICC/IF: LNCaP cells. Flow Cyt (intra): LNCaP cells.

  • General notes

    ab242397 is the carrier-free version of ab155076.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PKN3 antibody [EPR3511(2)] - BSA and Azide free (ab242397)
    Immunocytochemistry/ Immunofluorescence - Anti-PKN3 antibody [EPR3511(2)] - BSA and Azide free (ab242397)

    Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling PKN3 with Purified ab155076 at 1:300 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155076)

     

  • Flow Cytometry - Anti-PKN3 antibody [EPR3511(2)] - BSA and Azide free (ab242397)
    Flow Cytometry - Anti-PKN3 antibody [EPR3511(2)] - BSA and Azide free (ab242397)

    Flow Cytometry analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling PKN3 with Purified ab155076 at 1:300 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155076)

     

  • Anti-PKN3 antibody [EPR3511(2)] - BSA and Azide free (ab242397)
    Anti-PKN3 antibody [EPR3511(2)] - BSA and Azide free (ab242397)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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