Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20918-13] to Casein Kinase 1 gamma 2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free
    See all Casein Kinase 1 gamma 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20918-13] to Casein Kinase 1 gamma 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, WBmore details
    Unsuitable for: ICC/IF,IHC-Fr or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with human Casein Kinase 1 gamma 2; HEK-293T transfected with mouse Casein Kinase 1 gamma 2; Mouse and rat testis lysate. Flow Cyt (intra): MEF and HEK-293T cells. IP: Mouse testis lysate.

  • General notes

    ab270040 is the carrier-free version of ab238121.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
    Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized MEF (Mouse embryonic fibroblast (immortalized) cells labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238121).

  • Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
    Immunoprecipitation - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)

    Casein Kinase 1 gamma 2 was immunoprecipitated from 0.35 mg mouse testis tissue lysate with ab238121 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab238121 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse testis tissue lysate 10 ug

    Lane 2: ab238121 IP in mouse testis tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab238121 in mouse testis tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238121).

  • Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
    Flow Cytometry - Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized HEK-293T (Human embryonic kidney epithelial cell) cells (transfected with myc-tagged Casein Kinase 1 gamma 2 construct ) labelling Casein Kinase 1 gamma 2 with ab238121 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238121).

  • Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)
    Anti-Casein Kinase 1 gamma 2 antibody [EPR20918-13] - BSA and Azide free (ab270040)

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