Anti-SPAK (phospho S309) antibody (ab192798)
Key features and details
- Rabbit polyclonal to SPAK (phospho S309)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPAK (phospho S309) antibody
See all SPAK primary antibodies -
Description
Rabbit polyclonal to SPAK (phospho S309) -
Host species
Rabbit -
Specificity
ab192798 detects endogenous levels of SPAK only when phosphorylated at Serine 311.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human SPAK (phospho S309) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of Serine 311(S-F-R(p)-K-L).
Database link: Q9UEW8 -
Positive control
- COLO cell extract; Human brain tissue.
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General notes
This product was previously labelled as STK39
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab192798 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPAK (phospho S309) antibody (ab192798)All lanes : Anti-SPAK (phospho S309) antibody (ab192798) at 1/500 dilution
Lane 1 : COLO cell extract
Lane 2 : COLO cell extract with antigen-specific peptide
Predicted band size: 59 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAK (phospho S309) antibody (ab192798)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling SPAK (phospho S311) with ab192798 at 1/50 (left) or ab192798 preincubated with blocking peptide (right).
