Anti-SPAK antibody (ab106936)
Key features and details
- Goat polyclonal to SPAK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPAK antibody
See all SPAK primary antibodies -
Description
Goat polyclonal to SPAK -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog
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Immunogen
Synthetic peptide corresponding to Human SPAK aa 401-414 (internal sequence).
Sequence:C-SQEKSRRVKEENPE
Database link: NP_037365.2 -
Positive control
- WB: Human cerebellum cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPAK antibody (ab106936)All lanes : Anti-SPAK antibody (ab106936) at 1 µg/ml
Lane 1 : mouse brain tissue lysate
Lane 2 : rat brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
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Western blot - Anti-SPAK antibody (ab106936)Anti-SPAK antibody (ab106936) at 0.5 µg/ml + Jurkat lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPAK antibody (ab106936)ab106936 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex shows textured staining in the neurons. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
