Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IER3IP1 antibody [EPR13440] (ab181247)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13440] to IER3IP1
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IER3IP1 antibody [EPR13440]
    See all IER3IP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13440] to IER3IP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, HeLa and Human fetal heart lysates; Human kidney and Human cardiac muscle tissues; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13440

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Western blot - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    All lanes : Anti-IER3IP1 antibody [EPR13440] (ab181247) at 1/1000 dilution

    Lane 1 : HepG2 lysate
    Lane 2 : HeLa lysate
    Lane 3 : Human fetal heart lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 9 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Immunocytochemistry/ Immunofluorescence - Anti-IER3IP1 antibody [EPR13440] (ab181247)

    Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling IER3IP1 with ab181247 at 1/250 dilution, dollowed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and DAPI staining (blue).

  • Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Immunoprecipitation - Anti-IER3IP1 antibody [EPR13440] (ab181247)

    Western blot analysis of immunoprecipitation pellet from HeLa cell lysate  immunoprecipitated using ab181247 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000.

  • Flow Cytometry - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Flow Cytometry - Anti-IER3IP1 antibody [EPR13440] (ab181247)

    Flow Cytometricl analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling IER3IP1 with ab181247 at 1/210 (red) compared to a negative control antibody (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] (ab181247)

    Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling IER3IP1 with ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IER3IP1 antibody [EPR13440] (ab181247)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling IER3IP1 with ab181247 at 1/100 dilution, followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-IER3IP1 antibody [EPR13440] (ab181247)
    Anti-IER3IP1 antibody [EPR13440] (ab181247)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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