Recombinant human VEGF 165A protein (Active) (ab219883)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, ELISA
Preparation and Storage
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Images
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ELISA - Recombinant human VEGF 165A protein (Active) (ab219883)Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 µg/mL (100 µL/well) can bind Human VEGF165, His Tag with a linear range of 0.2-4 ng/mL.
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Functional Studies - Recombinant human VEGF 165A protein (Active) (ab219883)Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human VEGF165 Protein, His Tag with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200).
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SDS-PAGE - Recombinant human VEGF 165A protein (Active) (ab219883)Human VEGF165, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
