Recombinant human VEGF 165A protein (Active) (Biotin) (ab168684)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 100 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.
Images
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Functional Studies - Recombinant human VEGF 165A protein (Biotin) (ab168684)
Immobilized human VEGFR1/R2, Fc tag at 2μg/mL (100 μL/well) binds ab168684.
Linear range: 0.39-3.1 ng/mL (QC tested).
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Functional Studies - Recombinant human VEGF 165A protein (Biotin) (ab168684)Immobilized Human Neuropilin-1, His Tag at 2 μg/mL (100 μL/well) binds ab168684.
Linear range: 5-40 ng/mL (Routinely tested).
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Functional Studies - Recombinant human VEGF 165A protein (Biotin) (ab168684)Immobilized Anti-VEGF MAb,Human IgG1 (Avastin) at 5μg/mL (100 μL/well) binds ab168684.
Linear range: 0.24-1.95 ng/mL (Routinely tested).
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SDS-PAGE - Recombinant human VEGF 165A protein (Biotin) (ab168684)Reduced (Lane 1) and non-reduced (Lane 2) ab168684 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >95%.
As a result of glycosylation, the protein migrates as 25-30 kDa (monomer) under reducing condition, and 43-50 kDa under non-reducing condition.
