Anti-Rb (phospho S780) antibody (ab131264)
Key features and details
- Rabbit polyclonal to Rb (phospho S780)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Rb (phospho S780) antibody
See all Rb primary antibodies -
Description
Rabbit polyclonal to Rb (phospho S780) -
Host species
Rabbit -
Specificity
ab131264 detects endogenous levels of Rb only when phosphorylated at serine 780. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of Serine 780 (
T-L-S(p)-P-I
) of Human Rb (UniProt P06400). -
Positive control
- 3T3 cell extract treated with UV; HeLa cells; Human breast carcinoma tissue
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab131264 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Rb (phospho S780) with ab131264 at 1/50 dilution. Right panel was preincubated with blocking peptide.
-
All lanes : Anti-Rb (phospho S780) antibody (ab131264) at 1/500 dilution
Lane 1 : 3T3 cell extract untreated
Lane 2 : 3T3 cell extract treated with UV
Predicted band size: 106 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
-
Immunofluorescence analysis of methanol-fixed HeLa cells labelling Rb (phospho S780) with ab131264 at 1/100 dilution.