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Cancer Cell cycle Cell cycle inhibitors Rb

Anti-Rb (phospho S780) antibody (ab131264)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-Rb (phospho S780) antibody (ab131264)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Rb (phospho S780)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Rb (phospho S780) antibody
    See all Rb primary antibodies
  • Description

    Rabbit polyclonal to Rb (phospho S780)
  • Host species

    Rabbit
  • Specificity

    ab131264 detects endogenous levels of Rb only when phosphorylated at serine 780.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of Serine 780 (

    T-L-S(p)-P-I

    ) of Human Rb (UniProt P06400).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 3T3 cell extract treated with UV; HeLa cells; Human breast carcinoma tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab131264 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody (ab131264)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody (ab131264)
    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Rb (phospho S780) with ab131264 at 1/50 dilution. Right panel was preincubated with blocking peptide.
  • Western blot - Anti-Rb (phospho S780) antibody (ab131264)
    Western blot - Anti-Rb (phospho S780) antibody (ab131264)
    All lanes : Anti-Rb (phospho S780) antibody (ab131264) at 1/500 dilution

    Lane 1 : 3T3 cell extract untreated
    Lane 2 : 3T3 cell extract treated with UV

    Predicted band size: 106 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho S780) antibody (ab131264)
    Immunocytochemistry/ Immunofluorescence - Anti-Rb (phospho S780) antibody (ab131264)
    Immunofluorescence analysis of methanol-fixed HeLa cells labelling Rb (phospho S780) with ab131264 at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Anti-Rb (phospho S807) antibody [EPR17732] (ab184796)

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