Anti-Rb (phospho S780) antibody (ab47763)
Key features and details
- Rabbit polyclonal to Rb (phospho S780)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Rb (phospho S780) antibody
See all Rb primary antibodies -
Description
Rabbit polyclonal to Rb (phospho S780) -
Host species
Rabbit -
Specificity
This antibody detects Rb only when phosphorylated at Serine 780. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Rb (phospho S780).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rb (phospho S780) antibody (ab47763)
ab47763 at 1/50 dilution staining paraffin-embedded human breast carcinoma; left without and right with immunising peptide.
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Western blot - Anti-Rb (phospho S780) antibody (ab47763)All lanes : Anti-Rb (phospho S780) antibody (ab47763) at 1/500 dilution
Lane 1 : K562 cell extract treated with serum for 20 hours
Lane 2 : K562 cell extract
Predicted band size: 106 kDa
Typically 5-30ug of total protein was loaded per lane of the gel. -
Western blot - Anti-Rb (phospho S780) antibody (ab47763)Western blot analysis of extracts from K562 cells treated or untreated with serum using Rb antibody and ab47763.
