Anti-BFL-1/GRS antibody [SP435] (ab246319)
![Anti-BFL-1/GRS antibody [SP435] (ab246319)](https://www.abcam.com/ps/products/246/ab246319/Images/ab246319-349547-anti-bcl2a1-grs-antibody-sp435-immunohistochemistry-b-cell-lymphoma-human-.jpg "Anti-BFL-1/GRS antibody [SP435] (ab246319)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP435] to BFL-1/GRS
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-BFL-1/GRS antibody [SP435]
See all BFL-1/GRS primary antibodies -
Description
Rabbit monoclonal [SP435] to BFL-1/GRS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- IHC-P: Human B-cell lymphoma tissue.
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General notes
FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP435 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BFL-1/GRS antibody [SP435] (ab246319)
IHC image of ab246319 staining BFL-1/GRS in a section of formalin-fixed paraffin-embedded human B-cell lymphoma* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 30mins. The section was then incubated with ab246319, 1/117 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Anti-BFL-1/GRS antibody [SP435] (ab246319)](https://www.abcam.com/ps/products/246/ab246319/Images/ab246319-2-benefits-of-recombinant-antibodies.png)
Anti-BFL-1/GRS antibody [SP435] (ab246319)
