Anti-Cullin 4A/CUL-4A antibody (ab72548)
Key features and details
- Rabbit polyclonal to Cullin 4A/CUL-4A
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cullin 4A/CUL-4A antibody
See all Cullin 4A/CUL-4A primary antibodies -
Description
Rabbit polyclonal to Cullin 4A/CUL-4A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Chicken, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Sea urchin, Platypus
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Immunogen
Synthetic peptide corresponding to Human Cullin 4A/CUL-4A (C terminal).
Database link: (NP_001008895.1 -
General notes
This product was previously labelled as Cullin 4a, Cullin 4A/B
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cullin 4A/CUL-4A antibody (ab72548)All lanes : Anti-Cullin 4A/CUL-4A antibody (ab72548) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 88 kDa
Observed band size: 88 kDa
Additional bands at: 117 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody (ab72548)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling Cullin 4A/CUL-4A with ab72548 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Cullin 4A/CUL-4A antibody (ab72548)Detection of Human Cullin 4A/CUL-4A by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72548 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody (ab72548)IHC image of ab72548 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab72548, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
