Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3198] to Cullin 4A/CUL-4A
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-Cullin 4A/CUL-4A antibody [EPR3198]
See all Cullin 4A/CUL-4A primary antibodies
Description

Rabbit monoclonal [EPR3198] to Cullin 4A/CUL-4A
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Cullin 4A/CUL-4A aa 700-800 (C terminal). The exact sequence is proprietary.
Database link: Q13619
Positive control
- WB: HeLa, A375 and 293T cell lysates. IHC-P: Human breast carcinoma tissue.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product was previously labelled as Cullin 4a, Cullin 4A/B.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3198
Isotype

IgG
Research areas

Western blot - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

All lanes : Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554) at 1/20000 dilution

Lane 1 : Hela cell lysate
Lane 2 : A375 cell lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 88 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

ab92554 at 1/100 dilution staining Cullin 4A/CUL-4A in Human breast carcinoma by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

ab92554 showing positive staining in Hepatocellular carcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

ab92554 showing positive staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

ab92554 showing positive staining in Ovarian carcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Anti-Cullin 4A/CUL-4A antibody [EPR3198] (ab92554)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com