Anti-Skp1 antibody (ab228637)
Key features and details
- Rabbit polyclonal to Skp1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Skp1 antibody
See all Skp1 primary antibodies -
Description
Rabbit polyclonal to Skp1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Chicken, Guinea pig, Cow, Dog, Xenopus laevis, Zebrafish, Rhesus monkey
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Immunogen
Recombinant fragment within Human Skp1 (internal sequence). The exact sequence is proprietary.
Database link: P63208 -
Positive control
- WB: NIH/3T3, A549, NCI-H1299, HCT 116 and MCF7 whole cell lysates. IHC-P: Mouse middle brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Skp1 antibody (ab228637)
Paraffin-embedded mouse middle brain tissue stained for Skp1 with ab228637 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Skp1 antibody (ab228637)All lanes : Anti-Skp1 antibody (ab228637) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 19 kDa12% SDS-PAGE
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Western blot - Anti-Skp1 antibody (ab228637)Anti-Skp1 antibody (ab228637) at 1/3000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 19 kDa12% SDS-PAGE
