Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Skp1 antibody [EPR3304] (ab76502)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3304] to Skp1
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Skp1 antibody [EPR3304]
    See all Skp1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3304] to Skp1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Skp1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa, PC-12 and NIH/3T3 cell lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Skp1 antibody [EPR3304] (ab76502)
    Western blot - Anti-Skp1 antibody [EPR3304] (ab76502)
    All lanes : Anti-Skp1 antibody [EPR3304] (ab76502) at 1/2000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP labeled IgG at 1/2000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Flow Cytometry - Anti-Skp1 antibody [EPR3304] (ab76502)
    Flow Cytometry - Anti-Skp1 antibody [EPR3304] (ab76502)
    Overlay histogram showing HeLa cells stained with ab76502 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76502, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-Skp1 antibody [EPR3304] (ab76502)
    Anti-Skp1 antibody [EPR3304] (ab76502)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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