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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes SCF Complex E3 Ligase

Anti-FBXL21/FBL3 antibody (ab222894)

Anti-FBXL21/FBL3 antibody (ab222894)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to FBXL21/FBL3
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FBXL21/FBL3 antibody
  • Description

    Rabbit polyclonal to FBXL21/FBL3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Sheep
  • Immunogen

    Recombinant fragment corresponding to Human FBXL21/FBL3 aa 50-150.
    Database link: Q9UKT6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse heart and skeletal muscle lysates. IHC: Human lung cancer tissue. ICC/IF: HepG2 cells.
  • General notes

     This product was previously labelled as FBXL21

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • SCF Complex E3 Ligase
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • F Box E3 Ligase

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL21/FBL3 antibody (ab222894)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FBXL21/FBL3 antibody (ab222894)

    Paraffin-embedded human lung cancer tissue stained for FBXL21/FBL3 using ab222894 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-FBXL21/FBL3 antibody (ab222894)
    Immunocytochemistry/ Immunofluorescence - Anti-FBXL21/FBL3 antibody (ab222894)

    HepG2 (human liver hepatocellular carcinoma cell line) cells stained for FBXL21/FBL3 (green) using ab222894 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L) .

  • Western blot - Anti-FBXL21/FBL3 antibody (ab222894)
    Western blot - Anti-FBXL21/FBL3 antibody (ab222894)
    All lanes : Anti-FBXL21/FBL3 antibody (ab222894) at 1/1000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse skeletal muscle lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 49 kDa
    Observed band size: 49 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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