Anti-Skp1 antibody (ab233484)
Key features and details
- Rabbit polyclonal to Skp1
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Skp1 antibody
See all Skp1 primary antibodies -
Description
Rabbit polyclonal to Skp1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Mouse, Chicken, Guinea pig, Cow, Xenopus laevis, Cynomolgus monkey, Orangutan
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Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human Skp1 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P63208 -
Positive control
- IHC-P: Human cerebrum, glioma, liver and kidney tissues. WB: HEK-293T cell lysate; Rat and pig brain lysates; Recombinant human Skp1 protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab233484 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Skp1 antibody (ab233484)
Paraffin-embedded human cerebrum tissue stained for Skp1 using ab233484 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Skp1 antibody (ab233484)Paraffin-embedded human glioma tissue stained for Skp1 using ab233484 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Skp1 antibody (ab233484)Paraffin-embedded human liver tissue stained for Skp1 using ab233484 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Skp1 antibody (ab233484)Paraffin-embedded human kidney tissue stained for Skp1 using ab233484 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Skp1 antibody (ab233484)Anti-Skp1 antibody (ab233484) at 2 µg/ml + Recombinant human Skp1 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 19 kDa
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Western blot - Anti-Skp1 antibody (ab233484)All lanes : Anti-Skp1 antibody (ab233484) at 2 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Pig brain lysate
Lane 3 : Rat brainl lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 19 kDa
