Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Skp1 antibody [EPR3303] (ab80586)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3303] to Skp1
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Skp1 antibody [EPR3303]
    See all Skp1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3303] to Skp1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Skp1. The exact sequence is proprietary.

  • Positive control

    • WB: lysates from 293, HeLa, PC12 or 3T3 cells; IHC-P: Human brain tissue; Flow Cyt: permeabilized HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Skp1 antibody [EPR3303] (ab80586)
    Western blot - Anti-Skp1 antibody [EPR3303] (ab80586)
    All lanes : Anti-Skp1 antibody [EPR3303] (ab80586) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Skp1 antibody [EPR3303] (ab80586)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Skp1 antibody [EPR3303] (ab80586)

    Immunohistochemistry analysis of paraffin-embedded Human brain tissue with 1/100 ab80586.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Skp1 antibody [EPR3303] (ab80586)
    Flow Cytometry - Anti-Skp1 antibody [EPR3303] (ab80586)
    ab80586 at 1/50 dilution (red) staining Skp1 in permeabilized HeLa cells by Flow cytometry. Rabbit IgG (negative) is shown in green.
  • Anti-Skp1 antibody [EPR3303] (ab80586)
    Anti-Skp1 antibody [EPR3303] (ab80586)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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