Anti-Skp1 antibody [EPR3303] (ab80586)
![Anti-Skp1 antibody [EPR3303] (ab80586)](https://www.abcam.com/ps/products/80/ab80586/Images/ab80586-95874-anti-skp1-antibody-epr3303-western-blot.jpg "Anti-Skp1 antibody [EPR3303] (ab80586)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3303] to Skp1
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Skp1 antibody [EPR3303]
See all Skp1 primary antibodies -
Description
Rabbit monoclonal [EPR3303] to Skp1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human Skp1. The exact sequence is proprietary.
-
Positive control
- WB: lysates from 293, HeLa, PC12 or 3T3 cells; IHC-P: Human brain tissue; Flow Cyt: permeabilized HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3303 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Skp1 antibody [EPR3303] (ab80586)All lanes : Anti-Skp1 antibody [EPR3303] (ab80586) at 1/5000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Skp1 antibody [EPR3303] (ab80586)](https://www.abcam.com/ps/products/80/ab80586/Images/ab80586-95873-anti-skp1-antibody-epr3303-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Skp1 antibody [EPR3303] (ab80586)Immunohistochemistry analysis of paraffin-embedded Human brain tissue with 1/100 ab80586.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Flow Cytometry - Anti-Skp1 antibody [EPR3303] (ab80586)](https://www.abcam.com/ps/products/80/ab80586/Images/ab80586-95872-anti-skp1-antibody-epr3303-flow-cytometry.jpg)
Flow Cytometry - Anti-Skp1 antibody [EPR3303] (ab80586)ab80586 at 1/50 dilution (red) staining Skp1 in permeabilized HeLa cells by Flow cytometry. Rabbit IgG (negative) is shown in green. -
![Anti-Skp1 antibody [EPR3303] (ab80586)](https://www.abcam.com/ps/products/80/ab80586/Images/ab80586-4-benefits-of-recombinant-antibodies.png)
Anti-Skp1 antibody [EPR3303] (ab80586)
