Anti-ARHGAP4 antibody (ab226835)
Key features and details
- Rabbit polyclonal to ARHGAP4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ARHGAP4 antibody
See all ARHGAP4 primary antibodies -
Description
Rabbit polyclonal to ARHGAP4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human ARHGAP4 aa 350-400. The exact sequence is proprietary.
Database link: P98171 -
Positive control
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226835 was affinity purified using an epitope specific to ARHGAP4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ARHGAP4 antibody (ab226835)All lanes : Anti-ARHGAP4 antibody (ab226835) at 0.1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 secondsLysates were prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-ARHGAP4 antibody (ab226835)ARHGAP4 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226835 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226835 at 0.4 µg/ml.
Lane 1: ab226835 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
