Bouin’s Fixative, paraffin-embedded mouse backskin tissue stained for DNA/RNA Damage using ab62623 (1 hr at RT) at 1/100 dilution in immunohistochemical analysis.

Secondary Antibody: FITC Goat Anti-Mouse (green) at 1/50 for 1 hour at RT.

Mouse hepatocytes stained for DNA/RNA Damage (green) using ab62623 at 1/500 dilution in ICC/IF, followed by Alexa-Fluor^®488 conjugated Goat Anti-Mouse IgG (H+L).

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Left panel: ab62623 (1/1000 for 16 hours at RT) staining in ischemic rat brain tissue (fresh samples).
Center panel: DAPI staining
Right panel: merged

Secondary Antibody: Alexa Fluor^®546 Goat Anti-mouse (Red) at 1/500 for 1 hour at RT.

Immunohistochemical analysis of PFA-fixed paraffin-embedded rat femural tissue, labeling with ab62623 at a dilution of 1/50 incubated for 13 hours at 4°C in 1% BSA in TBS. Heat mediated antigen retrival was performed via Tris-EDTA pH 9.0. Blocking was via ab93695 ABC kit incubated at 1% for 20 minutes at room temperature. A secondary was not used, but ab93695 detection kit was used for signal amplification.

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ab62623 staining in rat liver tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with Proteinase K solution and then blocking for 20 minutes at 37°C was performed. The primary antibody was diluted 1/4000 and incubated with sample for 2 hours at 37°C. A Biotin conjugated rabbit polyclonal to mouse IgG was used as secondary antibody at 1/200 dilution.

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Paraffin-embedded mouse inflammed colon tissue stained for DNA/RNA Damage using ab62623 at 1/1,000,000 dilution (12 hrs at 4°C) in immunohistochemical analysis.

Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.