Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)
![Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)](https://www.abcam.com/ps/products/109/ab109005/Images/ab109005-265081-anti-sumo-23-antibody-epr4602-immunofluorescence.jpg "Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4602] to Sumo 2 + Sumo 3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
-
Product name
Anti-Sumo 2 + Sumo 3 antibody [EPR4602]
See all Sumo 2 + Sumo 3 primary antibodies -
Description
Rabbit monoclonal [EPR4602] to Sumo 2 + Sumo 3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
-
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, Jurkat, 293T, SW480 and HL60 cell lysates; Human colonic and hepatocellular carcinoma tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4602 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)
Immunocytochemistry/Immunofluorescence analysis of SW480 (human colorectal adenocarcinoma) cells labelling Sumo 2+3 (green) with purified ab109005 at 1/400. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
-
![Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)](https://www.abcam.com/ps/products/109/ab109005/Images/ab109005-121559-anti-sumo-23-antibody-epr4602-western-blot.jpg)
Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)All lanes : Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : SW480 cell lysate
Lane 5 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)](https://www.abcam.com/ps/products/109/ab109005/Images/ab109005-121558-anti-sumo-23-antibody-epr4602-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)ab109005 at 1/100 dilution, staining Sumo 2+3 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)](https://www.abcam.com/ps/products/109/ab109005/Images/ab109005-121557-anti-sumo-23-antibody-epr4602-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)ab109005 at 1/100 diltuion, staining Sumo 2+3 in Human hepatocellular carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)](https://www.abcam.com/ps/products/109/ab109005/Images/ab109005-451139-anti-sumo-2-sumo-3-antibody-epr4602-western-blot-recombinant-human.jpg)
Western blot - Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)All lanes : Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005) at 1/1000 dilution
Lane 1 :Recombinant Human Sumo 1 protein (ab140417) at 0.01 µg
Lane 2 :Recombinant Human Sumo 2 protein (ab140420) at 0.01 µg
Lane 3 :Recombinant Human Sumo 3 protein (ab140414) at 0.01 µg
Lane 4 :Recombinant Human Sumo 4 protein (ab157025) at 0.1 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking buffer: 5% NFDM/TBST
-
![Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)](https://www.abcam.com/ps/products/109/ab109005/Images/ab109005-5-benefits-of-recombinant-antibodies.png)
Anti-Sumo 2 + Sumo 3 antibody [EPR4602] (ab109005)
