Anti-MMP9 antibody [5G3] (ab119906) at 1/500 dilution + recombinant Human MMP9 protein (238-465 amino acids)


Predicted band size : 50.6 kDa

All lanes : Anti-MMP9 antibody [5G3] (ab119906) at 1/10000 dilution

Lane 1 : Jurkat WCL (negative control)
Lane 2 : Molt-4 WCL (negative control)
Lane 3 : U937 WCL (positive control)
Lane 4 : Human Tonsil (positive control)

Developed using the ECL technique.

Performed under reducing conditions.

Observed band size: 82,92 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.


Exposure time: 5 seconds

All lanes : Anti-MMP9 antibody [5G3] (ab119906) at 1/500 dilution

Lane 1 : HEK293 cell lysate
Lane 2 : MMP7-hIgGFc transfected HEK293 cell lysate
Lane 3 : MMP9-hIgGFc transfected HEK293 cell lysate


Predicted band size : 50.6 kDa

Red: Control Antigen (100ng);
Purple: Antigen (10ng);
Green: Antigen (50ng);
Blue: Antigen (100ng);