Anti-WIPI2 antibody - C-terminal (ab229225)
Key features and details
- Rabbit polyclonal to WIPI2 - C-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-WIPI2 antibody - C-terminal
See all WIPI2 primary antibodies -
Description
Rabbit polyclonal to WIPI2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human WIPI2 (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q9Y4P8 -
Positive control
- WB: K562, THP-1 and Jurkat whole cell extracts. IHC-P: Rat liver tissue. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-WIPI2 antibody - C-terminal (ab229225)All lanes : Anti-WIPI2 antibody - C-terminal (ab229225) at 1/2000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell extract
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 49 kDa10% SDS-PAGE gel.
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Western blot - Anti-WIPI2 antibody - C-terminal (ab229225)Anti-WIPI2 antibody - C-terminal (ab229225) at 1/2000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 49 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WIPI2 antibody - C-terminal (ab229225)Paraffin-embedded rat liver tissue stained for WIPI2 using ab229225 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-WIPI2 antibody - C-terminal (ab229225)Ice-cold methanol-fixed A431 (human epidermoid carcinoma cell line) cells stained for WIPI2 (green) using ab229225 at 1/2500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
