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Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3206(2)] to Galectin 1 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free
    See all Galectin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3206(2)] to Galectin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T cell lysate. IHC-P: Human kidney, skin, stomach, spleen, placenta, heart and brain tissues.

  • General notes

    Ab240111 is the carrier-free version of ab138513. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240111 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
    Western blot - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
    All lanes : Anti-Galectin 1 antibody [EPR3206(2)] (ab138513) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : LGALS1 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa



    This data was developed using the same antibody clone in a different buffer formation (ab138513).

    Lanes 1- 2: Merged signal (red and green). Green - ab138513 observed at 15 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab138513 was shown to react with Galectin 1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266850 (knockout cell lysate ab256974) was used. Wild-type HEK-293T and LGALS1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab138513 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Galectin 1 with ab138513 antibody at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138513).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)

    Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Galectin 1 with ab138513 antibody at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138513).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)

    Immunohistochemical analysis of paraffin embedded Normal Human Stomach tissue using ab138513 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138513).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)

    Immunohistochemical analysis of paraffin embedded Normal Human Spleen tissue using ab138513 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138513).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)

    Immunohistochemical analysis of paraffin embedded Normal Human Placenta tissue using ab138513 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138513).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)

    Immunohistochemical analysis of paraffin embedded Normal Human Heart tissue using ab138513 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138513).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)

    Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab138513 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138513).

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)
    Anti-Galectin 1 antibody [EPR3206(2)] - BSA and Azide free (ab240111)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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