Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Zika Virus NS1 antibody [B4] (ab218546)

Key features and details

  • Mouse monoclonal [B4] to Zika Virus NS1
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Zika virus
  • Isotype: IgG1

Overview

  • Product name

    Anti-Zika Virus NS1 antibody [B4]
    See all Zika Virus NS1 primary antibodies

  • Description

    Mouse monoclonal [B4] to Zika Virus NS1

  • Host species

    Mouse

  • Specificity

    ab218546 is specific for the NS1 protein of Zika virus, detecting NS1 from both the Uganda and Suriname strains. It demonstrates negligible cross-reactivity with NS1 proteins from Dengue virus (all serotypes), Japanese Encephalitis Virus and Yellow Fever Virus. A small amount of cross-reactivity has been observed with the NS1 protein from West Nile Virus in a direct ELISA.

  • Tested applications

    Suitable for: WB, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: Zika virus

  • Immunogen

    Recombinant full length protein corresponding to Zika Virus NS1. Produced in HEK-293 cells.
    Database link: Q32ZE1

  • Positive control

    • Recombinant Zika Virus NS1; supernatant from Zika Virus infected Vero cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7
    Constituent: 100% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    ab218546 was purified from hybridoma cell culture supernatant by affinity chromatography on Protein G. >95% pure by SDS-PAGE.

  • Clonality

    Monoclonal

  • Clone number

    B4

  • Isotype

    IgG1

Images

  • Western blot - Anti-Zika Virus NS1 antibody [B4] (ab218546)
    Western blot - Anti-Zika Virus NS1 antibody [B4] (ab218546)
    All lanes : Anti-Zika Virus NS1 antibody [B4] (ab218546) at 1 µg/ml

    Lane 1 : Zika virus NS1 at 0.1 µg
    Lane 2 : Zika virus NS1 at 0.02 µg
    Lane 3 : Zika virus NS1 at 0.004 µg
    Lane 4 : Zika virus NS1 at 0.0008 µg
    Lane 5 : Dengue virus serotype 1 at 0.1 µg
    Lane 6 : Dengue virus serotype 2 at 0.1 µg
    Lane 7 : Dengue virus serotype 3 at 0.1 µg
    Lane 8 : Dengue virus serotype 4 at 0.1 µg
    Lane 9 : West Nile virus NS1 NY99 at 0.1 µg
    Lane 10 : Supernatant of Zika virus infected Vero cells at 5 µl

    Secondary
    All lanes : Goat anti-Mouse IgG HRP conjugated


    4-12% Bis-Tris gel

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