Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SDHC antibody [EPR11035(B)] (ab155999)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11035(B)] to SDHC
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SDHC antibody [EPR11035(B)]
    See all SDHC primary antibodies

  • Description

    Rabbit monoclonal [EPR11035(B)] to SDHC

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human SDHC (UniProt Q99643).

  • Positive control

    • THP-1, HeLa and fetal liver lysates; Human liver and lung carcinoma tissues; rat liver lysates; THP-1; Mouse brain lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (ab155999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (ab155999)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling SDHC with purified  ab155999 at 1:700 dilution (0.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-SDHC antibody [EPR11035(B)] (ab155999)
    Western blot - Anti-SDHC antibody [EPR11035(B)] (ab155999)
    All lanes : Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/10000 dilution (unpurified)

    Lane 1 : THP-1 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (ab155999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (ab155999)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling SDHC with unpurified ab155999 at 1/250 dilution.

  • Western blot - Anti-SDHC antibody [EPR11035(B)] (ab155999)
    Western blot - Anti-SDHC antibody [EPR11035(B)] (ab155999)
    Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/50000 dilution (purified) + Rat liver lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 19 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-SDHC antibody [EPR11035(B)] (ab155999)
    Western blot - Anti-SDHC antibody [EPR11035(B)] (ab155999)
    All lanes : Anti-SDHC antibody [EPR11035(B)] (ab155999) at 1/10000 dilution (purified)

    Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
    Lane 2 : Human fetal liver lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (ab155999)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] (ab155999)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling SDHC with unpurified ab155999 at 1/250 dilution.

  • Anti-SDHC antibody [EPR11035(B)] (ab155999)
    Anti-SDHC antibody [EPR11035(B)] (ab155999)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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