Canine NGAL ELISA Kit (ab205085)
Key features and details
- Sensitivity: 0.01 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine NGAL ELISA Kit
See all Lipocalin-2 / NGAL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.01 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 15.6pg/ml - 1000pg/ml -
Assay time
3h 20m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
NGAL (ab205085) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of NGAL protein in biological samples of dogs.
In this assay the NGAL present in samples reacts with the anti-NGAL antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, the Detection Antibody, biotin conjugated anti-NGAL, is added and complexes are formed. Following a wash step, the horseradish peroxidase (HRP) conjugated Streptavidin is added and complexes are formed. After another washing step, the complexes are assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of NGAL in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of NGAL in the test sample. The quantity of NGAL in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Detection Antibody Conjugate 1 x 150µl 100X HRP Streptavidin 1 x 150µl 1X Diluent Concentrate 1 x 60ml 20X Wash Buffer Concentrate 1 x 50ml Anti-Dog NGAL ELISA Microplate 1 unit Chromogen Substrate Solution 1 x 12ml DOG NGAL Calibrator (Lyophilized) 1 vial Stop Solution 1 x 12ml -
Research areas
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Function
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth. -
Tissue specificity
Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized. - Information by UniProt
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Alternative names
- 24p3
- 25 kDa alpha 2 microglobulin related subunit of MMP9
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
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Images
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Standard Curve.
Representative standard curve using ab205085 Dog NGAL ELISA Kit.