Anti-Melanoma gp100 antibody (ab52058)
Key features and details
- Goat polyclonal to Melanoma gp100
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Melanoma gp100 antibody
See all Melanoma gp100 primary antibodies -
Description
Goat polyclonal to Melanoma gp100 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
CPIGENSPLLSGQQ
, corresponding to C terminal amino acids 647-660 of Human Melanoma gp100 -
Positive control
- Human skin lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Melanoma gp100 antibody (ab52058)Anti-Melanoma gp100 antibody (ab52058) at 1 µg/ml + Human Skin lysate at 35 µg
Predicted band size: 70 kDa
Additional bands at: 26 kDa (possible immature (unprocessed))Primary incubation was 1 hour. Detected by chemiluminescence. The 26kDa band observed in Human Skin lysate corresponds to the precusor protein M-beta fragment (Leonhardt et al, Mol Biol Cell. 2013 Apr;24(7):964-81.PMID: 23389629).
