Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP4863(2)] to Melanoma gp100 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, Flow Cyt, ICC
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free
    See all Melanoma gp100 primary antibodies

  • Description

    Rabbit monoclonal [EP4863(2)] to Melanoma gp100 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, Flow Cyt, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysate and Human melanoma lysate. IHC: Human melanoma tissue. ICC: B16-F0 (Mouse melanoma epithelial cell-like) cell line. Flow cyt: B16-F0 (Mouse melanoma epithelial cell-like) cell line. IP: B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysate.

  • General notes

    ab256497 is the carrier-free version of ab137078 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab256497 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunoprecipitation - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)
    Immunoprecipitation - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)

    ab137078 immunoprecipitating Melanoma gp100. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

    Lane 1: B16-F0 (mouse melanoma epithelial cell-like) whole cell lysate 10ug
    Lane 2: B16-F0 (mouse melanoma epithelial cell-like) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab137078 in B16-F0 whole cell lysate

    Exposure time: 1 second

    Observed MW: 85-100 KDa

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Western blot - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)
    Western blot - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)
    Anti-Melanoma gp100 antibody [EP4863(2)] (ab137078) at 1/10000 dilution + B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 70 kDa



    Exposure time: 50 seconds

    Observed MW: 85-100 KDa

    The molecular weights observed are consistent with what have been described in PMID: 16492709 and PMID: 7961886

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Western blot - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)
    Western blot - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)
    Anti-Melanoma gp100 antibody [EP4863(2)] (ab137078) at 1/1000 dilution + Human melanoma lysate 15µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 70 kDa



    Exposure time: 18-0 seconds

    Observed MW: 85-100 KDa

    The molecular weights observed are consistent with what have been described in PMID: 16492709 and PMID: 7961886

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)

    ab137078 staining Melanoma gp100 in human melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250 for 30 minutes at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

     

  • Immunocytochemistry - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)
    Immunocytochemistry - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)

    ab137078 staining Melanoma gp100 in B16-F0 (mouse skin) cells by ICC (Immunocytochemistry). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) ab195889 was used as a counterstain at a dilution of 1/200. DAPI was used as a nuclear counterstain.

     This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Flow Cytometry - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)
    Flow Cytometry - Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)

    Overlay histogram showing B16-F0 (Mouse skin melanoma cell) cells stained with ab137078 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 90% methanol. The cells were then incubated with ab137078 at 1/40 dilution. The secondary antibody used wasGoat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution. Isotype control antibody (black line) was Rabbit monoclonal IgG (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)
    Anti-Melanoma gp100 antibody [EP4863(2)] - BSA and Azide free (ab256497)

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