Anti-Ym-1 + Ym-2 antibody [EPR15263] - BSA and Azide free (ab227110)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15263] to Ym-1 + Ym-2 - BSA and Azide free
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Ym-1 + Ym-2 antibody [EPR15263] - BSA and Azide free
See all Ym-1 + Ym-2 primary antibodies -
Description
Rabbit monoclonal [EPR15263] to Ym-1 + Ym-2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lung, mouse brain, mouse heart, C6, Raw264.7, PC-12 and NIH3T3 lysates. IHC-P: Mouse liver tissue. ICC/IF: Raw264.7 cells Flow Cyt: C2C12 cells.
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General notes
Ab227110 is the carrier-free version of ab192029. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab227110 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15263 -
Isotype
IgG -
Research areas
Images
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Flow Cytometrical analysis of paraformaldehyde-fixed C2C12 cells labeling Chitinase 3 like protein 3 + Chitinase 3 with ab192029 at 1/200 compared to a negative control cell. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192029).
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Immunofluorescent analysis of paraformaldehyde-fixed Raw264.7 cells labeling Chitinase 3 like protein 3 + Chitinase 3 like protein 4 with ab192029 at 1/200, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 (ab150077) and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-Chitinase 3 like protein 3 + Chitinase 3 like protein 4(Mouse) ) is 1/200 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution; 2. Primary ab concentration (anti-Chitinase 3 like protein 3 + Chitinase 3 like protein 4(Mouse) ) is 1/200 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab192029).
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This IHC data was generated using the same anti-Ym1/2 antibody clone, EPR15263, in a different buffer formulation (cat# ab192029).
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Chitinase 3 like protein 3 + Chitinase 3 like protein 4 with ab192029 at 1/500 with prediluted HRP polymer for Rabbit IgG as secondary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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