Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15263] to Ym-1 + Ym-2
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Ym-1 + Ym-2 antibody [EPR15263]
    See all Ym-1 + Ym-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR15263] to Ym-1 + Ym-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Mouse
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung, mouse brain, mouse heart, C6, Raw264.7, PC-12 and NIH3T3 lysates. IHC-P: Mouse liver tissue. ICC/IF: Raw264.7 cells Flow Cyt: C2C12 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15263

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)
    Immunocytochemistry/ Immunofluorescence - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

    Immunofluorescent analysis of paraformaldehyde-fixed Raw264.7 cells labeling Chitinase 3 like protein 3 + Chitinase 3 like protein 4 with ab192029 at 1/200, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 (ab150077) and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-Chitinase 3 like protein 3 + Chitinase 3 like protein 4(Mouse) ) is 1/200 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution; 2. Primary ab concentration (anti-Chitinase 3 like protein 3 + Chitinase 3 like protein 4(Mouse) ) is 1/200 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Chitinase 3 like protein 3 + Chitinase 3 like protein 4 with ab192029 at 1/500 with prediluted HRP polymer for Rabbit IgG as secondary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)
    Flow Cytometry - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

    Flow Cytometrical analysis of paraformaldehyde-fixed C2C12 cells labeling Chitinase 3 like protein 3 + Chitinase 3 with ab192029 at 1/200 compared to a negative control cell. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Western blot - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)
    Western blot - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)
    All lanes : Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029) at 1/10000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : C6 lysate
    Lane 4 : Raw264.7 lysate
    Lane 5 : PC-12 lysate
    Lane 6 : NIH3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44, 45 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)
    Western blot - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)
    Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029) at 1/50000 dilution + Mouse lung lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44, 45 kDa
    Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.

  • Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)
    Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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