Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15263] to Ym-1 + Ym-2
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat

Product name

Anti-Ym-1 + Ym-2 antibody [EPR15263]
See all Ym-1 + Ym-2 primary antibodies
Description

Rabbit monoclonal [EPR15263] to Ym-1 + Ym-2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-P	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse lung, mouse brain, mouse heart, C6, Raw264.7, PC-12 and NIH3T3 lysates. IHC-P: Mouse liver tissue. ICC/IF: Raw264.7 cells Flow Cyt: C2C12 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15263
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

Immunofluorescent analysis of paraformaldehyde-fixed Raw264.7 cells labeling Chitinase 3 like protein 3 + Chitinase 3 like protein 4 with ab192029 at 1/200, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 (ab150077) and DAPI staining (blue). The two negative controls: 1. Primary ab concentration (anti-Chitinase 3 like protein 3 + Chitinase 3 like protein 4(Mouse) ) is 1/200 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution; 2. Primary ab concentration (anti-Chitinase 3 like protein 3 + Chitinase 3 like protein 4(Mouse) ) is 1/200 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Chitinase 3 like protein 3 + Chitinase 3 like protein 4 with ab192029 at 1/500 with prediluted HRP polymer for Rabbit IgG as secondary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

Flow Cytometrical analysis of paraformaldehyde-fixed C2C12 cells labeling Chitinase 3 like protein 3 + Chitinase 3 with ab192029 at 1/200 compared to a negative control cell. FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

All lanes : Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029) at 1/10000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : C6 lysate
Lane 4 : Raw264.7 lysate
Lane 5 : PC-12 lysate
Lane 6 : NIH3T3 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44, 45 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029) at 1/50000 dilution + Mouse lung lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44, 45 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Anti-Ym-1 + Ym-2 antibody [EPR15263] (ab192029)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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