Anti-Cdc23 antibody [EPR11827] (ab177148)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11827] to Cdc23
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-Cdc23 antibody [EPR11827]
See all Cdc23 primary antibodies -
Description
Rabbit monoclonal [EPR11827] to Cdc23 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human Cdc23 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9UJX2 -
Positive control
- HepG2, HeLa, K562 and Jurkat cell lysates. HeLa cells.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11827 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cdc23 antibody [EPR11827] (ab177148) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 68 kDa
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Immunofluorescent analysis of HeLa cells labeling Cdc23 with ab177148 at 1/50 dilution.
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Western blot analysis on immunoprecipitation pellet from (Lane 1) K562 cells lysate or (Lane 2) 1X PBS (negative control) using ab177148 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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