Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOCS1 antibody [EPR24290-356] (ab280886)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24290-356] to SOCS1
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SOCS1 antibody [EPR24290-356]
    See all SOCS1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24290-356] to SOCS1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: KARPAS-299, K562, Mouse thymus lysates. Flow Cyt: KARPAS-299 cells. IP: KARPAS-299 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
    Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
    All lanes : Anti-SOCS1 antibody [EPR24290-356] (ab280886) at 1/1000 dilution

    Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 3 : U266B1 (human multiple myeloma B lymphocyte) whole cell lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 23 kDa
    Observed band size: 23,22 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:15580314, 26090662, 10490100).

    Negative control: U266B1 (PMID: 15580314).

    Exposure time: Lane 1: 48 seconds Lane 2-3: 3 minutes

     

  • Flow Cytometry - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
    Flow Cytometry - Anti-SOCS1 antibody [EPR24290-356] (ab280886)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U266B1 (human multiple myeloma B lymphocyte, left)/ KARPAS-299 (Human anaplastic large cell lymphoma, right) cells labelling SOCS1 with ab280886 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: U266B1 (PMID: 15580314).

  • Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
    Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] (ab280886)

    SOCS1 was immunoprecipitated from 0.35 mg of KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate with ab280886 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280886 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10 ug

    Lane 2: ab280886 IP in KARPAS-299 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab280886 in mouse eyeball tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 93 seconds

     

  • Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
    Western blot - Anti-SOCS1 antibody [EPR24290-356] (ab280886)
    Anti-SOCS1 antibody [EPR24290-356] (ab280886) at 1/1000 dilution + Mouse thymus tissue lysate at 60 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 15580314, 26090662, 10490100).

    Exposure time: 3 minutes

     

  • Anti-SOCS1 antibody [EPR24290-356] (ab280886)
    Anti-SOCS1 antibody [EPR24290-356] (ab280886)

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