Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15657] to Androgen receptor variant 5,6,7es
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Androgen receptor variant 5,6,7es antibody [EPR15657]
    See all Androgen receptor variant 5,6,7es primary antibodies

  • Description

    Rabbit monoclonal [EPR15657] to Androgen receptor variant 5,6,7es

  • Host species

    Rabbit

  • Specificity

    This ARv567es antibody (Ab200827, clone EPR15657) is a rabbit monoclonal antibody against Human Androgen receptor variant 5,6,7es (aa 700 to the C-terminus). This antibody does not react with AR-FL.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: M12-3FARV567es. IHC-P: Human prostate cancer tissue.

  • General notes

    Anti-ARv567es RabMAb product (Clone EPR15657) has been highlighted in the following poster: Identification of ARv567es expression profile in the prostate cancer clinical samples with a newly developed antibody.

    For ARv7 specific RabMAb antibody - see ab198394.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15657

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)

    Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen receptor variant 5,6,7es with ab200827 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on Human prostate cancer tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)
    Western blot - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)
    All lanes : Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 3 : LNCaP (Human prostate cancer cell line) whole cell lysate
    Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
    Lane 5 : Human prostate lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 77 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)
    Western blot - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)
    Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827) at 1/10000 dilution + M12-3FARV567es

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 77 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Full length ARV567es M12 overexpress lysate containing aa1-739 with 3× DDDDK-tag was supplied by Stephen R. Plymate, M.D. Professor, Department of Medicine, University of Washington, Seattle, WA.

     

    Liu G. et al. 2013. AR variant ARv567es induces carcinogenesis in a novel transgenic mouse model of prostate cancer. Neoplasia 15, 1009-1017.

     

    Cao B. et al. 2014. Androgen receptor splice variants activating the full-length receptor in mediating resistance to androgen-directed therapy. Oncotarget 5, 1646-1656.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)

    Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen receptor variant 5,6,7es with ab200827 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on Human prostate cancer tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)

    Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue labeling Androgen receptor variant 5,6,7es with ab200827 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative on Human prostate cancer. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Androgen receptor variant 5,6,7es with ab200827 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Negative on Human liver tissue. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)
    Anti-Androgen receptor variant 5,6,7es antibody [EPR15657] (ab200827)

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