Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y301] to GRB2 - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GRB2 antibody [Y301] - BSA and Azide free
    See all GRB2 primary antibodies

  • Description

    Rabbit monoclonal [Y301] to GRB2 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises GRB2. It can also detect the splice isoform GRB3-3 of GRB2.

  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt, WBmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247231 is the carrier-free version of ab32111. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247231 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    Western blot - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    All lanes : Anti-GRB2 antibody [Y301] (ab32111) at 1/5000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 5 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa
    Observed band size: 25 kDa



    This data was developed using ab32111, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    We are unsure how to define the extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    This data was developed using ab32111, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of GRB2 expression in paraffin embedded human breast carcinoma tissue section, using 1/100 ab32111. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    Immunocytochemistry - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    This data was developed using ab32111, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of GRB2 expression in PC12 cells, using 1/100 ab32111.
  • Flow Cytometry - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    Flow Cytometry - Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    This data was developed using ab32111, the same antibody clone in a different buffer formulation.Overlay histogram showing SH-SY5Y cells stained with ab32111 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32111, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)
    Anti-GRB2 antibody [Y301] - BSA and Azide free (ab247231)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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