Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20708] to NAGLU/NAG
Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-NAGLU/NAG antibody [EPR20708]
See all NAGLU/NAG primary antibodies
Description

Rabbit monoclonal [EPR20708] to NAGLU/NAG
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
ICC/IF	Mouse Human
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human placenta, liver and fetal kidney lysates; HepG2 whole cell lysate; Mouse colon and testis lysates; Rat colon, testis, liver and spleen lysates. IHC-P: Human kidney and liver tissues; Mouse and rat kidney tissues. ICC/IF: HepG2 and Hepa1-6 cells. Flow Cyt: Hepa1-6 and HepG2 cells.
General notes
This product was previously labelled as NAGLU

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20708
Isotype

IgG
Research areas

Western blot - Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Lanes 1-3 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/1000 dilution
Lane 4 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/5000 dilution

Lane 1 : Human placenta lysate at 20 µg
Lane 2 : Human liver lysate at 20 µg
Lane 3 : Human fetal kidney lysate at 10 µg
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 82 kDa
Observed band size: 82 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lanes 1-2: 1 minute; Lanes 3-4: 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on human kidney tubules (PMID: 4291567, PMID: 24244710). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Immunofluorescent analysis of 100% methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling NAGLU/NAG with ab214671 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Lanes 1-4 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/2000 dilution
Lanes 5-6 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/1000 dilution

Lane 1 : Mouse colon lysate at 20 µg
Lane 2 : Rat testis lysate at 20 µg
Lane 3 : Mouse testis lysate at 20 µg
Lane 4 : Rat colon lysate at 20 µg
Lane 5 : Rat liver lysate at 10 µg
Lane 6 : Rat spleen lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 82 kDa
Observed band size: 82 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lanes 1-2,5-6: 3 minutes; Lanes 3-4: 30 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on human liver (PMID: 4291567, PMID: 8776591). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on mouse kidney tubules (PMID: 4291567, PMID: 24244710). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on rat kidney tubules (PMID: 4291567, PMID: 24244710). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Immunofluorescent analysis of 100% methanol-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling NAGLU/NAG with ab214671 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Flow cytometric analysis of 4% paraformaldehyde-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling NAGLU/NAG with ab214671at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry - Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling NAGLU/NAG with ab214671at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Anti-NAGLU/NAG antibody [EPR20708] (ab214671)

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