Anti-Otx2 antibody (ab76748)
Key features and details
- Rabbit polyclonal to Otx2
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
-
Product name
Anti-Otx2 antibody
See all Otx2 primary antibodies -
Description
Rabbit polyclonal to Otx2 -
Host species
Rabbit -
Specificity
Reacts specifically with 31 kDa Otx2 protein. -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
-
Immunogen
Synthetic peptide derived from the N terminal domain of human Otx2
-
Positive control
- This antibody gave a positive siganl in OTX2 Overexpression Lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Otx2 antibody (ab76748)All lanes : Anti-Otx2 antibody (ab76748) at 1 µg/ml
Lane 1 : OTX1 Recombinant Protein
at 0.1 µg
Lane 2 : OTX2 Overexpression Lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Otx1 Recombinant Protein was used as a negative control. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab76748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
