Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RDH5 antibody [EPR15191] (ab200197)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15191] to RDH5
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RDH5 antibody [EPR15191]
    See all RDH5 primary antibodies

  • Description

    Rabbit monoclonal [EPR15191] to RDH5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IP
    Rat
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat eyeball lysates; Y79 cell lysate. ICC/IF: Y79 cells. IP: Rat eyeball whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15191

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RDH5 antibody [EPR15191] (ab200197)
    Western blot - Anti-RDH5 antibody [EPR15191] (ab200197)
    All lanes : Anti-RDH5 antibody [EPR15191] (ab200197) at 1/5000 dilution

    Lane 1 : Mouse eyeball lysate
    Lane 2 : Rat eyeball lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
    Lane 6 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RDH5 antibody [EPR15191] (ab200197)
    Western blot - Anti-RDH5 antibody [EPR15191] (ab200197)
    Anti-RDH5 antibody [EPR15191] (ab200197) at 1/200 dilution + Y79 (Human retinoblastoma cell line) cell lysate at 30 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-RDH5 antibody [EPR15191] (ab200197)
    Immunocytochemistry/ Immunofluorescence - Anti-RDH5 antibody [EPR15191] (ab200197)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Y79 (Human retinoblastoma cell line) cells labeling RDH5 with ab200197 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

    Cytoplasm staining on Y79 cell line is observed.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab200197 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Immunoprecipitation - Anti-RDH5 antibody [EPR15191] (ab200197)
    Immunoprecipitation - Anti-RDH5 antibody [EPR15191] (ab200197)

    RDH5 was immunoprecipitated from 1mg of Rat eyeball whole cell lysate with ab200197 at 1/30 dilution.

    Lane 1: Rat eyeball whole cell lysate 10ug (Input).

    Lane 2: ab200197 IP in Rat eyeball whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200197 in Rat eyeball whole cell lysate.

    Western blot was performed from the immunoprecipitate using ab200197 at 1/1000 dilution.

    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Anti-RDH5 antibody [EPR15191] (ab200197)
    Anti-RDH5 antibody [EPR15191] (ab200197)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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