Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 09, 2021

Anti-RDH5 antibody [EPR15191] (ab200197)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15191] to RDH5
Suitable for: WB, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-RDH5 antibody [EPR15191]
See all RDH5 primary antibodies
Description

Rabbit monoclonal [EPR15191] to RDH5
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IP	Rat
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat eyeball lysates; Y79 cell lysate. ICC/IF: Y79 cells. IP: Rat eyeball whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15191
Isotype

IgG
Research areas

Western blot - Anti-RDH5 antibody [EPR15191] (ab200197)

All lanes : Anti-RDH5 antibody [EPR15191] (ab200197) at 1/5000 dilution

Lane 1 : Mouse eyeball lysate
Lane 2 : Rat eyeball lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat spleen lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
Lane 6 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-RDH5 antibody [EPR15191] (ab200197)

Anti-RDH5 antibody [EPR15191] (ab200197) at 1/200 dilution + Y79 (Human retinoblastoma cell line) cell lysate at 30 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-RDH5 antibody [EPR15191] (ab200197)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Y79 (Human retinoblastoma cell line) cells labeling RDH5 with ab200197 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Cytoplasm staining on Y79 cell line is observed.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: ab200197 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunoprecipitation - Anti-RDH5 antibody [EPR15191] (ab200197)

RDH5 was immunoprecipitated from 1mg of Rat eyeball whole cell lysate with ab200197 at 1/30 dilution.
Lane 1: Rat eyeball whole cell lysate 10ug (Input).
Lane 2: ab200197 IP in Rat eyeball whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200197 in Rat eyeball whole cell lysate.
Western blot was performed from the immunoprecipitate using ab200197 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Anti-RDH5 antibody [EPR15191] (ab200197)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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