Anti-HuR / ELAVL1 antibody (ab28660)
Key features and details
- Rabbit polyclonal to HuR / ELAVL1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HuR / ELAVL1 antibody
See all HuR / ELAVL1 primary antibodies -
Description
Rabbit polyclonal to HuR / ELAVL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human HuR/ ELAVL1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab28843)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseChickenCowApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Another band of approximately 52 kDa is also detected, we are unsure as the identity of this band.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). Another band of approximately 52 kDa is also detected, we are unsure as the identity of this band.Target
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Function
Involved in 3'-UTR ARE-mediated MYC stabilization. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, HUR binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsMethylated at Arg-217 by CARM1 in macrophages in response to LPS challenge. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1994 Human
- Entrez Gene: 15568 Mouse
- Omim: 603466 Human
- SwissProt: Q15717 Human
- SwissProt: P70372 Mouse
- Unigene: 184492 Human
- Unigene: 713744 Human
- Unigene: 119162 Mouse
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Alternative names
- ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody
- ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody
- ELAV like 1 antibody
see all
Images
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Western blot - Anti-HuR / ELAVL1 antibody (ab28660)All lanes : Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.
Protocols
References (3)
ab28660 has been referenced in 3 publications.
- Green LC et al. Human antigen R as a therapeutic target in pathological cardiac hypertrophy. JCI Insight 4:N/A (2019). PubMed: 30668549
- Wang F et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). PubMed: 28244490
- Workman E et al. U1A regulates 3' processing of the survival motor neuron mRNA. J Biol Chem 289:3703-12 (2014). PubMed: 24362020
Images
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Western blot - Anti-HuR / ELAVL1 antibody (ab28660)All lanes : Anti-HuR / ELAVL1 antibody (ab28660) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.
