Human DROSHA knockout HEK-293T cell lysate (ab257171)
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260929 - Human DROSHA knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
-
Western blot - Human DROSHA knockout HEK293T cell lysate (ab257171)
Lane 1: Wild-type HEK293T cell lysate (20 µg)
Lane 2: DROSHA knockout HEK293T cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lanes 1-3: Merged signal (red and green). Green - ab183732 observed at 159 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab183732 Anti-Drosha antibody [EPR12794] was shown to specifically react with Drosha in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266217 (knockout cell lysate ab257171) was used. Wild-type and Drosha knockout samples were subjected to SDS-PAGE. ab183732 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
-
Sanger Sequencing - Human DROSHA knockout HEK293T cell lysate (ab257171)Homozygous: 1 bp insertion in exon 4
