Antibodies, Proteins, Kits and Reagents for Life Science

301 536 ₸ Product size

100 µl 301 536 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Mouse monoclonal [1H5D8] to CD14
Suitable for: ICC/IF, IHC-P, WB
Reacts with: Human
Isotype: IgG2a

Product name

Anti-CD14 antibody [1H5D8]
See all CD14 primary antibodies
Description

Mouse monoclonal [1H5D8] to CD14
Host species

Mouse

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CD14 aa 20-214. (Expressed in E.coli).
Sequence:
TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPF LKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLK ELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLK PGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMA

Database link: P08571
Run BLAST with Run BLAST with
Positive control
- WB: Human CD14 (aa 20-214) recombinant protein; CD14 (aa 20-214)-hIgGFc transfected HEK-293 cell lysate. IHC-P: Human cervical cancer and colon tissues. ICC/IF: HepG2 cells.
General notes

This product was changed from ascites to supernatant. Lot no’s high than GR281538-10 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS

Contains 0.5% protein stabilizer.
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

1H5D8
Isotype

IgG2a
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (ab181470)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD14 with ab181470 at 1/200 dilution with DAB staining.
Western blot - Anti-CD14 antibody [1H5D8] (ab181470)

All lanes : Anti-CD14 antibody [1H5D8] (ab181470) at 1/500 dilution

Lane 1 : Non-transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CD14 (aa 20-214)-hIgGFc transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Predicted band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-CD14 antibody [1H5D8] (ab181470)

Immunofluorescent analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling CD14 with ab181470 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [1H5D8] (ab181470)

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD14 with ab181470 at 1/200 dilution with DAB staining.
Western blot - Anti-CD14 antibody [1H5D8] (ab181470)

Anti-CD14 antibody [1H5D8] (ab181470) at 1/500 dilution + Human CD14 (aa 20-214) recombinant protein

Predicted band size: 40 kDa

Expected MWt is 46.8 kDa.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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