Anti-Piwi antibody (ab5207)
Key features and details
- Rabbit polyclonal to Piwi
- Suitable for: WB
- Reacts with: Drosophila melanogaster
- Isotype: IgG
Overview
-
Product name
Anti-Piwi antibody -
Description
Rabbit polyclonal to Piwi -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Drosophila melanogaster
-
Immunogen
Synthetic peptide corresponding to Drosophila melanogaster Piwi aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available asab14803) -
Positive control
- This antibody gave a positive signal in Drosophila embryo nuclear extract
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB Drosophila melanogasterApplication Abreviews Notes WB Notes Application notesWB: 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). Can be blocked with Piwi peptide (ab14803).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
-
Relevance
Piwi mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell. It is not essential for the further differentiation of the committed daughter cell. Acts cell autonomously to promote germline stem cell division. It is expressed somatically in the ovariole terminal filament and epithelial sheath cells. Expressed in the germarium at low levels during oogenesis stages 1-6, at a lower level during stages 7-9, strongly at stage 10 and eventually accumulating in early embryos. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 34521 Drosophila melanogaster
- SwissProt: Q9VKM1 Drosophila melanogaster
-
Alternative names
- P-element induced wimpy testis antibody
- Piwi antibody
Images
-
Western blot - Anti-Piwi antibody (ab5207)Anti-Piwi antibody (ab5207) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands. -
Western blot - Anti-Piwi antibody (ab5207)This image is courtesy of Ahmet DenliDrosophila embryo lysate, 8% TRIS SDS-PAGE.
Drosophila embryo lysate, 8% TRIS SDS-PAGE. The arrow indicates Piwi at a predicted MW of 90 kDa. Pre-immune sera was used as a control and showed no equivalent band (data not shown). Ahmet Denli
The arrow indicates Piwi at a predicted MW of 90 kDa.
Pre-immune sera was used as a control and showed no equivalent band (data not shown).
Datasheets and documents
References (8)
ab5207 has been referenced in 8 publications.
- Jiménez-Merino J et al. Putative stem cells in the hemolymph and in the intestinal submucosa of the solitary ascidian Styela plicata. Evodevo 10:31 (2019). PubMed: 31788180
- Jeffery WR Distal Regeneration Involves the Age Dependent Activity of Branchial Sac Stem Cells in the Ascidian Ciona intestinalis. Regeneration (Oxf) 2:1-18 (2015). PubMed: 25893097
- Patil VS et al. The Tudor domain protein Tapas, a homolog of the vertebrate Tdrd7, functions in the piRNA pathway to regulate retrotransposons in germline of Drosophila melanogaster. BMC Biol 12:61 (2014). WB ; Drosophila melanogaster . PubMed: 25287931
- Dufourt J et al. Spatio-temporal requirements for transposable element piRNA-mediated silencing during Drosophila oogenesis. Nucleic Acids Res N/A:N/A (2013). IHC-P ; Drosophila melanogaster . PubMed: 24288375
- Moshkovich N et al. RNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function. Genes Dev 25:1686-701 (2011). WB ; Drosophila melanogaster . PubMed: 21852534
- Klenov MS et al. Separation of stem cell maintenance and transposon silencing functions of Piwi protein. Proc Natl Acad Sci U S A 108:18760-5 (2011). PubMed: 22065765
- Brown FD et al. Whole body regeneration in a colonial ascidian, Botrylloides violaceus. J Exp Zool B Mol Dev Evol 312:885-900 (2009). PubMed: 19588490
- Rosner A et al. Vasa and the germ line lineage in a colonial urochordate. Dev Biol 331:113-28 (2009). WB . PubMed: 19406116
Images
-
Western blot - Anti-Piwi antibody (ab5207)Anti-Piwi antibody (ab5207) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.
-
Western blot - Anti-Piwi antibody (ab5207) This image is courtesy of Ahmet Denli
Drosophila embryo lysate, 8% TRIS SDS-PAGE.
Drosophila embryo lysate, 8% TRIS SDS-PAGE. The arrow indicates Piwi at a predicted MW of 90 kDa. Pre-immune sera was used as a control and showed no equivalent band (data not shown). Ahmet Denli
The arrow indicates Piwi at a predicted MW of 90 kDa.
Pre-immune sera was used as a control and showed no equivalent band (data not shown).