Anti-RPGR antibody (ab264373)
Key features and details
- Rabbit polyclonal to RPGR
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-RPGR antibody
See all RPGR primary antibodies -
Description
Rabbit polyclonal to RPGR -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human RPGR aa 825-875. The exact sequence is proprietary.
Database link: Q92834 -
Positive control
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-RPGR antibody (ab264373)All lanes : Anti-RPGR antibody (ab264373) at 0.04 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 113 kDa
Exposure time: 30 secondsLysates prepared in NETN lysis buffer.
-
Immunoprecipitation - Anti-RPGR antibody (ab264373)RPGR was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using ab264373 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264373 at 0.4 μg/ml.
Lane 1: ab264373 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Exposure time: 30 seconds.
Cells prepared using NETN lysis buffer.
