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Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)

Key features and details

  • Mouse monoclonal [OTI4E8] to ALDH1A3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free
    See all ALDH1A3 primary antibodies

  • Description

    Mouse monoclonal [OTI4E8] to ALDH1A3 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment corresponding to Human ALDH1A3. Amino acids 1-100 and 413-512.
    Database link: P47895

  • Positive control

    • WB: Transfected HEK-293T, A459 cell lysate; IHC-P: Human prostate and prostate carcinoma tissue; Flow Cyt (Intra): Jurkat whole cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    All lanes : Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651) at 1/500 dilution

    Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : pCMV6-ENTRY ALDH1A3-transfected HEK-293T whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 56 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)

    Immunohistochemical analysis of paraffin-embedded human prostate tissue staining ALDH1A3 with a mouse monoclonal ALDH1A3 antibody [OTI4E8].

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Flow Cytometry - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Flow Cytometry - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)

    Flow cytometric analysis of Jurkat cells using a mouse monoclonal ALDH1A3 antibody [OTI4E8] (Red), compared to a nonspecific negative control antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    All lanes : Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651) at 1/200 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : SV-T2 (Simian Virus 40-transformed BALB/c mouse embryo fibroblasts cell line) whole cell lysate
    Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 5 : COS-7 (african green monkey kidney fibroblast-like cell line) whole cell lysate
    Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 7 : MDCK (canine kidney cell line) whole cell lysate
    Lane 8 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 56 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)

    Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue staining ALDH1A3 with a mouse monoclonal ALDH1A3 antibody [OTI4E8].

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

  • Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    Western blot - Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651)
    All lanes : Anti-ALDH1A3 antibody [OTI4E8] - BSA and Azide free (ab273651) at 1/200 dilution

    Lane 1 : Negative control E. coli lysate
    Lane 2 : E. coli lysate containing human ALDH1A3 recombinant protein fragment gene

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 56 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

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