Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

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Anti-DUSP4 antibody [EPR19881] (ab216576)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19881] to DUSP4
Suitable for: ICC/IF, Flow Cyt, IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-DUSP4 antibody [EPR19881]
See all DUSP4 primary antibodies
Description

Rabbit monoclonal [EPR19881] to DUSP4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MDA-MB-231, A549, SK-BR-3, HCT 116, RAW 264.7, PC-12 and C6 whole cell lysates; Human breast cancer lysate. ICC/IF: A549 and MDA-MB-231 cells. Flow Cyt: MDA-MB-231 cells. IP: MDA-MB-231 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19881
Isotype

IgG
Research areas

Western blot - Anti-DUSP4 antibody [EPR19881] (ab216576)

All lanes : Anti-DUSP4 antibody [EPR19881] (ab216576) at 1/1000 dilution

Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate at 10 µg
Lane 3 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate at 10 µg
Lane 4 : HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 10 µg

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/2:3 minutes; Lane 3: 30 seconds; Lane 4: 1 second.
Immunocytochemistry/ Immunofluorescence - Anti-DUSP4 antibody [EPR19881] (ab216576)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling DUSP4 with ab216576 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on A549 cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Western blot - Anti-DUSP4 antibody [EPR19881] (ab216576)

Anti-DUSP4 antibody [EPR19881] (ab216576) at 1/1000 dilution + Human breast cancer lysate at 10 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-DUSP4 antibody [EPR19881] (ab216576)

All lanes : Anti-DUSP4 antibody [EPR19881] (ab216576) at 1/1000 dilution

Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa
Observed band size: 43 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1:3 minutes; Lane 2/3: 15 seconds.
Immunocytochemistry/ Immunofluorescence - Anti-DUSP4 antibody [EPR19881] (ab216576)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling DUSP4 with ab216576 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining on MDA-MB-231 cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Flow Cytometry - Anti-DUSP4 antibody [EPR19881] (ab216576)

Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling DUSP4 with ab216576 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-DUSP4 antibody [EPR19881] (ab216576)

DUSP4 was immunoprecipitated from 0.35mg of MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate with ab216576 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab216576 at 1/500 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution

Lane 1: MDA-MB-231 whole cell lysate, 10µg (Input).

Lane 2: ab216576 IP in MDA-MB-231 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216576 in MDA-MB-231 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-DUSP4 antibody [EPR19881] (ab216576)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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