Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19881] to DUSP4 - BSA and Azide free
  • Suitable for: ICC/IF, WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DUSP4 antibody [EPR19881] - BSA and Azide free
    See all DUSP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19881] to DUSP4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MDA-MB-231, A549, SK-BR-3, HCT 116, RAW 264.7, PC-12 and C6 whole cell lysates; Human breast cancer lysate. ICC/IF: A549 and MDA-MB-231 cells. Flow Cyt: MDA-MB-231 cells. IP: MDA-MB-231 whole cell lysate.

  • General notes

    Ab222487 is the carrier-free version of ab216576. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab222487 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)
    Immunocytochemistry/ Immunofluorescence - Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling DUSP4 with ab216576 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on A549 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216576).

  • Immunocytochemistry/ Immunofluorescence - Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)
    Immunocytochemistry/ Immunofluorescence - Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling DUSP4 with ab216576 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear staining on MDA-MB-231 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216576).

  • Flow Cytometry - Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)
    Flow Cytometry - Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)

    Flow cytometric analysis of 4% paraformaldehyde-fixed MDA-MB-231 (Human breast adenocarcinoma cell line) cells labeling DUSP4 with ab216576 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216576).

  • Immunoprecipitation - Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)
    Immunoprecipitation - Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)

    DUSP4 was immunoprecipitated from 0.35mg of MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate with ab216576 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab216576 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: MDA-MB-231 whole cell lysate, 10µg (Input).

    Lane 2: ab216576 IP in MDA-MB-231 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216576 in MDA-MB-231 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab216576).

  • Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)
    Anti-DUSP4 antibody [EPR19881] - BSA and Azide free (ab222487)

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