Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP170] to PTEN - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB
- Reacts with: Human
Overview
-
Product name
Anti-PTEN antibody [SP170] - BSA and Azide free
See all PTEN primary antibodies -
Description
Rabbit monoclonal [SP170] to PTEN - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Dog -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: MCF7 cell lysate.Flow Cyt: A431 cells.IHC-P: Human prostate, ovary, prostate adenocarcinoma, colon adenocarcinoma, stomach adenocarcinoma, cervical squamous cell carcinoma, lung squamous cell carcinoma and ovarian carcinoma tissues.
-
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
ab272009 is the carrier-free version of ab227657. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SP170 -
Isotype
IgG -
Research areas
Images
-
Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657). -
Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657). -
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657). -
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657). -
Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling PTEN with ab227657 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657). -
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657). -
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657). -