Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP170] to PTEN - BSA and Azide free
Suitable for: IHC-P, Flow Cyt, WB
Reacts with: Human

Product name

Anti-PTEN antibody [SP170] - BSA and Azide free
See all PTEN primary antibodies
Description

Rabbit monoclonal [SP170] to PTEN - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, Flow Cyt, WBmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Dog
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7 cell lysate.Flow Cyt: A431 cells.IHC-P: Human prostate, ovary, prostate adenocarcinoma, colon adenocarcinoma, stomach adenocarcinoma, cervical squamous cell carcinoma, lung squamous cell carcinoma and ovarian carcinoma tissues.
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
ab272009 is the carrier-free version of ab227657. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm.

Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

SP170
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human ovary tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Flow Cytometry - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling PTEN with ab227657 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Formalin-fixed, paraffin-embedded human prostate tissue stained for PTEN using ab227657 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227657).
Anti-PTEN antibody [SP170] - BSA and Azide free (ab272009)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com