Anti-PNUTS antibody (ab70247)
Key features and details
- Rabbit polyclonal to PNUTS
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PNUTS antibody
See all PNUTS primary antibodies -
Description
Rabbit polyclonal to PNUTS -
Host species
Rabbit -
Specificity
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Other Immunogen Type corresponding to PNUTS aa 350-400.
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Positive control
- Nuclear extract from Hela cells
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General notes
This product was previously labelled as PPP1R10
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to PNUTS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNUTS antibody (ab70247)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling PNUTS with ab70247 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-PNUTS antibody (ab70247)Lanes 1-2 : Anti-PNUTS antibody (ab70247) at 1 µg/ml
Lanes 3-4 : Anti-PNUTS antibody (ab70247) at 0.2 µg/ml
Lanes 1 & 3 : Nuclear extract from Hela cells at 15 µg
Lanes 2 & 4 : Nuclear extract from Hela cells at 45 µg
Predicted band size: 99 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-PNUTS antibody (ab70247)Immunoprecipitation/ Western Blot of PNUTS Lane 1: Another antibody which recognizes a different epitope of PNUTS. Lane 2: Another antibody which recognizes a different epitope of PNUTS. Lane 3: ab70247 at 1µg/100µg nuclear extract. Lane 4: Another antibody which recognizes a different epitope of PNUTS. Detection: Chemiluminescence with exposure times of 3 seconds.
